THE 2-MINUTE RULE FOR AGX51

The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products had been digested and cloned right into a pET21a+ vector using NdeI and XhoI restriction enzymes. A point mutation was released by the short transform web page-directed mutagenesis pr